PURExpressIn Vitro Protein Synthesis Kit from New England Biolabs

Product Specs
ItemPURExpressIn Vitro Protein Synthesis Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE6800L
Quantity100 reactions
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Machining water through laser cutting of nanoparticle-encased water pancakesNature CommunicationsJune 29, 2023Jicheng Niu, Wenjing Liu, Jasmine Xinze Li, Xianglong Pang, Yulin Liu, Chao Zhang, Keyang Yue, Yulin Zhou, Feng Xu, Xiaoguang Li, et al.expression system was performed using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The target RNA consisted of a 36nt sequence (GGG UGA UGG GAC AUU CCG AUG UCC CAU CAA UAA GAG CAA GAC

(2) CRISPR-induced DNA reorganization for multiplexed nucleic acid detectionNature CommunicationsMarch 17, 2023Margot Karlikow, Evan Amalfitano, Xiaolong Yang, Jennifer Doucet, Abigail Chapman, Peivand Sadat Mousavi, Paige Homme, Polina Sutyrina, Winston Chan, Sofia Lemak, et al.a 1X PBS solution to a final concentration of 20 µM before use. Cell-free system NEB PURExpress (#E6800L) was used as the only source of cell-free system (CFS) in this paper. Cell-free reactions where prepared

(3) Development of Toehold Switches as a Novel Ribodiagnostic Method for West Nile VirusGenes (Basel)January 16, 2023Antonis Giakountis, Zoe Stylianidou, Anxhela Zaka, Styliani Pappa, Anna Papa, Christos Hadjichristodoulou, Kostas D. MathiopoulosTranslation In vitro Transcription-Translation (IVT-TA) reactions were performed with PURExpress® kit (NEB #E6800L, Bioline Thessaloniki, Greece), as previously described [37], in the presence of 100ng WNV cultured

(4) PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide librariesNature ProtocolsNovember 16, 2022Sierra N. Henson, Evan A. Elko, Piotr M. Swiderski, Yong Liang, Anna L. Engelbrektson, Alejandra Piña, Annalee S. Boyle, Zane Fink, Salvatore J. Facista, Vidal Martinez, et al.reagents PURExpress in vitro protein synthesis kit (store at −80 °C; New England Biolabs, cat. no. E6800L): Critical! Construct sequences and peptide-encoding algorithm have been designed for performance with

(5) FIDELITY: A quality control system for droplet microfluidicsScience AdvancesJuly 8, 2022Han Zhang, Can Huang, Yuwen Li, Rohit Gupte, Ryan Samuel, Jing Dai, Adrian Guzman, Rushant Sabnis, Paul de Figueiredo, Arum Hanthe DNA template for New England Biolabs (NEB) PURExpress in vitro protein synthesis reagent (NEB, #E6800L). Droplet generation (80 μm) and PCR amplification were carried out using the protocol described by

(6) COVID-19 vaccination elicits an evolving, cross-reactive antibody response to epitopes conserved with endemic coronavirus spike proteinsCell ReportsJuly 5, 2022Evan A. Elko et al.Transcription Kit,Lucigen,Cat# ASF3507 PURExpress In Vitro Protein Synthesis Kit,New England Biolabs,Cat# E6800L SCoV-2 Detect™ IgG,InBios,Cat# COVE-G SARS-CoV-2 Surrogate Virus Neutralization Test,GenScript,Cat#

(7) Comprehensive analysis of PNA-based antisense antibiotics targeting various essential genes in uropathogenic Escherichia coliNucleic Acids ResearchJune 24, 2022Linda Popella, Jakob Jung, Phuong Thao Do, Regan J Hayward, Lars Barquist, Jörg Vogeltarget gene-gfp fusion constructs, the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described in the instructions with minor modifications. To ensure a precisely adjusted

(8) Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samplesNature Biomedical EngineeringMarch 7, 2022Margot Karlikow, Severino Jefferson Ribeiro da Silva, Yuxiu Guo, Seray Cicek, Larissa Krokovsky, Paige Homme, Yilin Xiong, Talia Xu, Maria-Angelica CalderÃ³n-PelÃ¡ez, Sigrid Camacho-Ortega, et al.for detection of amplified target(s)15. Cell-free reactions Cell-free reactions (PURExpress, NEB, E6800L) were assembled following the manufacturer’s instructions, as described previously15. Reactions were

(9) Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assayLife Science AllianceDecember 1, 2021Anirudh Chakravarthy, Anirudh Nandakumar, Geen George, Shyamsundar Ranganathan, Suchitta Umashankar, Nishan Shettigar, Dasaradhi Palakodeti, Akash Gulyani, Arati Rameshnuclease free water for further use. IVTT assay Cell-free IVTT reactions (NEB PURExpress, Cat. no. E6800L) were prepared using the manufacturer’s protocol. In a total reaction volume of 10μl, 4 μl of solution

(10) Residue-by-residue analysis of cotranslational membrane protein integration in vivoeLifeFebruary 8, 2021Felix Nicolaus, Ane Metola, Daphne Mermans, Amanda Liljenström, Ajda Krč, Salmo Mohammed Abdullahi, Matthew Zimmer, Thomas F Miller III, Gunnar von Heijnelinear fragments for in vitro expression "Commercial assay, kit",PURExpress,New England Biolabs,Cat# E6800L,Used for in vitro expression "Chemical compound, drug",35S methionine,PerkinElmer,Cat# NEG009T001MC,35S

(11) Epitope-resolved profiling of the SARS-CoV-2 antibody response identifies cross-reactivity with endemic human coronavirusesCell Reports MedicineJanuary 19, 2021Jason T. Ladner, Sierra N. Henson, Annalee S. Boyle, Anna L. Engelbrektson, Zane W. Fink, Fatima Rahee, Jonathan D’ambrozio, Kurt E. Schaecher, Mars Stone, Wenjuan Dong, et al.Transcription Kit,Lucigen,Cat# ASF3507 PURExpress In Vitro Protein Synthesis Kit,New England Biolabs,Cat# E6800L ,, Deposited data,Deposited data,Deposited data ,, PepSeq read count data,Open Science Framework,https://osf.io/ak2tm/ ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, "Custom

(12) A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodiesbioRxiv : the preprint server for biologyOctober 30, 2020Chen X, Gentili M, Hacohen N, Regev A.in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes, then placed on ice, and 200 μl ice cold stop buffer

(13) Precise and Programmable Detection of Mutations Using Ultraspecific RiboregulatorsCellMarch 5, 2020Fan Hong, Duo Ma, Kaiyue Wu, Lida A. Mina, Rebecca C. Luiten, Yan Liu, Hao Yan, Alexander A. Greenreactions were conducted using PURExpress in vitro protein synthesis systems (New England Biolabs, E6800L). In a typical experiment, the total volume for the reaction was 6 μL. Solutions A and B were combined

(14) PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free SystemsACS Synthetic BiologyMay 17, 2019Scott Wick, David I. Walsh, Johanna Bobrow, Kimberly Hamad-Schifferli, David S. Kong, Todd Thorsen, Keri Mroszczyk, Peter A. CarrMaster Mix Solution Components of the PURExpress cell-free synthesis kit (New England Biolabs, part #E6800L) were thawed on ice. Fluorescent molecule stock tubes for ReAsH-EDT2 labeling reagent (2 mM in DMSO;

(15) A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkersNature CommunicationsDecember 1, 2018Melissa K. Takahashi, Xiao Tan, Aaron J. Dy, Dana Braff, Reid T. Akana, Yoshikazu Furuta, Nina Donghia, Ashwin Ananthakrishnan, James J. Collinsreactions were performed using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The cell-free reactions consisted of NEB Solution A (40%), NEB Solution B (30%), RNase inhibitor (0.5%;

(16) Nascent peptide assists the ribosome in recognizing chemically distinct small moleculesNature Chemical BiologyMarch 1, 2016Pulkit Gupta, Bo Liu, Dorota Klepacki, Vrinda Gupta, Klaus Schulten, Alexander S. Mankin, Nora Vázquez-Laslopvolume of 5 μl of PURExpress cell-free transcription-translation system (New England Biolabs, Cat. No. E6800L) following the manufacturer’s protocol. When required, ERY or TEL were added to the final concentration

(17) Paper-Based Synthetic Gene NetworksCellNovember 6, 2014Keith Pardee, Alexander A. Green, Tom Ferrante, D. Ewen Cameron, Ajay DaleyKeyser, Peng Yin, James J. Collinsexpressing constitutive TetR (0.05) to reduce promoter leakage. For the PT7 cell-free system (NEB, E6800L), solution A (0.4) and B (0.3) were combined together, with the remaining volume comprised of RNase

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